| Catalog Number: TD-26925 |
| Gene Symbol: ARL3 |
| Description: Anti active Arl3 Mouse Monoclonal Antibody |
| Background: Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery. |
| Immunogen: Recombinant Arl3 protein (active form) |
| Applications: IP, IHC, IF |
| Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 、1:50~100 dilution for IHC, IF |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Arl3 antibody recognizes active Arl3-GTP from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
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Arl3 Activation Assay. Purified His-tagged Arl3 proteins were immunoprecipitated with the anti active Arl3 monoclonal antibody (Cat. #TD-26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl3 monoclonal antibody . Input controls are shown in the bottom panel. |