

| Catalog Number: TD-26922 | |
| Gene Symbol: RAB35 | |
| Description: Anti Active Rab35 Mouse Monoclonal Antibody | |
| Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. | |
| Immunogen: Recombinant full length protein of active Rab35 | |
| Applications: IP, IHC, IF | |
| Recommended Dilutions: 1 µg for ~2 mg total cellular proteins for IP 、1:100 dilution for IHC, IF | |
| Concentration: 1 mg/ml | |
| Host: mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Storage buffer: | |
| Preservative: no | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti active Rab35 antibody recognizes active Rab35 proteins of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | |
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Immunoprecipitation/Western blot:
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Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti active Rab35 monoclonal antibody (Cat. No. TD-26922). Immunoblot was with an anti Rab35 polyclonal antibody. |
