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Anti Active Ran Mouse Monoclonal Antibody
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TD-26915
- Catalog： TD-26915
- Clonality： 单克隆
- Application： IP, IHC, IF
- Synonyms： Active Ran-GTP
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规格：
30ul
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价格：
¥4800
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Ran-GTP
| Cat. #: TD-26915 | |
| Size: 30 µL | |
| Gene Symbol: RAN | |
| Description: Anti-Ran-GTP Mouse Monoclonal Antibody | |
| Background: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. | |
| Immunogen: Recombinant full length protein of active Ran. | |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) | |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins、IHC, IF: 1:50-1:250 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-Ran-GTP monoclonal antibody recognizes active Ran of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid freeze/thaw cycles | |
|
Immunoprecipitation/Western blot: |
Ran Activation Assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Ran-GTP monoclonal antibody (Cat. No. TD-26915). Immunoblot was with an anti-Ran polyclonal antibody. |