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Anti Active Rab5 Mouse Monoclonal Antibody
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TD-26911
- Catalog： TD-26911
- Clonality： 单克隆
- Application： IP, IHC, IF
- Synonyms： Active Rab5-GTP
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规格：
30ul
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价格：
¥4800
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Rab5-GTP
| Cat. #: TD-26911 | |
| Size: 30 µL | |
| Gene Symbol: RAB5 | |
| Description: Anti-Rab5-GTP Monoclonal Antibody | |
| Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. | |
| Immunogen: Recombinant full length protein of active Gao | |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) | |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG1 | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
| Immunoprecipitation/Western blot:
|
Rab5 Activation Assay.Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rab5-GTP monoclonal antibody (Cat. No. TD-26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. ). |