| Catalog Number: TD-26924 |
| Gene Symbol: Arl1 |
| Description: Anti active Arl1 Mouse Monoclonal Antibody |
| Background: Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal. |
| Immunogen: Recombinant Arl1 protein (active form) |
| Applications: IP, IHC, IF |
| Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 、1:50~100 dilution for IHC, IF |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Arl1 antibody recognizes active Arl1-GTP from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
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Arl1 Activation Assay. Purified GST-tagged Arl1 proteins were immunoprecipitated with the anti active Arl1 monoclonal antibody (Cat. # TD-26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl1 monoclonal antibody. Arl1 protein input control is shown in the bottom panel. |