Rab35,Rab35-GTP

Activation Assay Kit

Configuration-specific monoclonal antibody that recognizes Rab35-GTP, but not Rab35-GDP The only available Rab35 activation assay kit directly measures the level of Rab35-GTP Providing shorter experimental time Could be used to monitor the activation of Rab35 in cells and in tissues by immunohistochemistry

Tanda Biosciences Rab35 Activation Assay Kit bases on the configuration-specific anti-Rab35-GTP monoclonal antibody to measure the active Rab35-GTP levels, either from cell extracts or from in vitro GTPγS loading Rab35 activation assays. Briefly, anti-active Rab35 mouse monoclonal antibody will be incubated with cell lysates containing Rab35-GTP. The bound active Rab35 will then be pulled down by protein A/G agarose. The precipitated active Rab35 will be detected by immunoblot analysis using anti- Rab35 rabbit polyclonal antibody.

The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. Currently there is no direct assay to measure the activation of Rab35 GTPases. Tanda Biosciences Rab35 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab35-GTP, but not Rab Rab35-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility. These anti- Rab35-GTP monoclonal antibodies can also be used to monitor the activation of Rab35 in cells and in tissues by immunohistochemistry. Tanda Biosciences Rab35 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab35. Each kit provides sufficient quantities to perform 30 assays.

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