RheB,RheB-GTP

Activation Assay Kit

Configuration-specific monoclonal antibody that recognizes RheB-GTP, but not RheB-GDP The only available RheB activation assay kit directly measures the level of RheB-GTP Providing shorter experimental time Could be used to monitor the activation of RheB in cells and in tissues by immunohistochemistry

Tanda Biosciences RheB Activation Assay Kit bases on the configuration-specific anti-RheB-GTP monoclonal antibody to measure the active RheB-GTP levels, either from cell extracts or from in vitro GTP?S loading RheB activation assays. Briefly, anti-active RheB mouse monoclonal antibody will be incubated with cell lysates containing RheB-GTP. The bound active RheB will then be pulled down by protein A/G agarose. The precipitated active RheB will be detected by immunoblot analysis using anti- RheB rabbit polyclonal antibody.

Small GTPases are a super-family of cellular signaling regulators. RheB is a member of the Ras-superfamily GTPases. RheB has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related. Currently there is no direct assay to measure the activation of RheB GTPases. Tanda Biosciences RheB Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes RheB-GTP, but not RheB-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility. These anti-RheB-GTP monoclonal antibody can also be used to monitor the activation of RheB in cells and in tissues by immunohistochemistry. Tanda Biosciences RheB Activation Assay Kit provides a simple and fast method to monitor the activation of RheB. Each kit provides sufficient quantities to perform 30 assays.

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