Ran,Ran-GTP

Activation Assay Kit

Configuration-specific monoclonal antibody that recognizes Ran-GTP, but not Ran-GDP The only available Ran activation assay kit directly measures the level of Ran-GTP Providing reliable results with consistent reproducibility than other Ran activation assay kits based on certain downstream signaling pathways Providing shorter experimental time Could be used to monitor the activation of Ran in cells and in tissues by immunohistochemistry

Tanda Biosciences Ran Activation Assay Kit bases on the configuration-specific anti-Ran-GTP monoclonal antibody to measure the active Ran-GTP levels, either from cell extracts or from in vitro GTPΓS loading Ran activation assays. Briefly, anti-active Ran mouse monoclonal antibody will be incubated with cell lysates containing Ran-GTP. The bound active Ran will then be pulled down by protein A/G agarose. The precipitated active Ran will be detected by immunoblot analysis using anti-Ran rabbit polyclonal antibody.

Small GTPases are a super-family of cellular signaling regulators. Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. Currently the activation of Ran proteins is assayed with the binding of GTP-bound Ran to the Ran-binding domain (RBD) of Raf protein kinase. This method is based on the observation that the active, GTP-bound Ran could bind to the RBD of Raf. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of RBD to Ran-GTP. Currently there is no direct assay to measure the activation of Ran GTPases. Tanda Biosciences Ran Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ran-GTP, but not Ran-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility. These anti-Ran-GTP monoclonal antibodies can also be used to monitor the activation of Ran in cells and in tissues by immunohistochemistry. Tanda Biosciences Ran Activation Assay Kit provides a simple and fast method to monitor the activation of Ran. Each kit provides sufficient quantities to perform 30 assays.

store at -20℃