TD-JN894012

Recombinant Treponema pallidum/TP TPP15 & tmpA & TPP47 Protein, C-His(TD-JN894012) expressed inE. coli. Purity:>90%by SDS-PAGE.HighlightsHis-Tagged— N-terminal 6×His tag for IMAC purification.E. coli Expression— High-yield, cost-effective production.High Purity— >90% purity verified by SDS-PAGE.

E. coli

P16055, P07643, P29723

TPP15 [Ser19-Arg141] + tmpA [Gly20-Arg345] + TPP47 [Gly21-Gln434]

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Treponema pallidum (strain Nichols)

Recombinant

Please contact with the lab for this information.

>90% as determined by SDS-PAGE.

114.54 kDa

Lyophilized

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.　　RECONSTITUTION PROTOCOL　　1.　Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. ２.　Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. ３.　Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. ４.　After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial.Note: Do not mix by vortexing or by pipetting the material up and down. ５.　Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. ６.　Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

15 kDa lipoprotein, Major membrane immunogen, tpp15, TP_0171, Treponemal membrane protein A, Antigen TmpA, Membrane protein A, tmpA, TP_0768, Putative DD-carboxypeptidase TP_0574, 3.4.-.-, 47 kDa lipoprotein, Tp47, Tpp47, 47 kDa membrane antigen, 47-kilodalton major integral membrane immunogen, TP_0574

SDS-PAGE for Recombinant Treponema pallidum/TP TPP15 & tmpA & TPP47 Protein, C-His

For research use only.