fT4 (Free Thyroxine) ELISA Kit

ELISA

For the determination of Human, Rat, Mouse fT4(Free Thyroxine) concentrations in serum, plasma and other related biological fluids.

This assay employs the competitive enzyme immunoassay technique. Determine the level of fT4(Free Thyroxine) in the sample. Add standard or sample to the microplate that have been pre-coated with fT4(Free Thyroxine) antigen. Add Biotin-conjugated anti-fT4(Free Thyroxine) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of fT4(Free Thyroxine) in the sample is inversely proportional to the OD value. The concentration of fT4(Free Thyroxine) in the sample can be calculated by drawing a standard curve.

0.94 pg/mL

1.56-100 pg/mL

This kit recognizes fT4(Free Thyroxine) in samples. No significant cross-reactivity or interference between fT4(Free Thyroxine) and analogues was observed.

Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.

A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.

Store at 2‑8 °C.

This kit is only for scientific research, not for use