25-HVD3 ELISA Kit

ELISA

For the determination of Human, Rat, Mouse 25-HVD3(25-Hydroxy Vitamin D3) concentrations in serum, plasma and other related biological fluids.

This assay employs the competitive enzyme immunoassay technique. Determine the level of 25-HVD3(25-Hydroxy Vitamin D3) in the sample. Add standard or sample to the microplate that have been pre-coated with 25-HVD3(25-Hydroxy Vitamin D3) antigen. Add Biotin-conjugated anti-25-HVD3(25-Hydroxy Vitamin D3) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of 25-HVD3(25-Hydroxy Vitamin D3) in the sample is inversely proportional to the OD value. The concentration of 25-HVD3(25-Hydroxy Vitamin D3) in the sample can be calculated by drawing a standard curve.

1.88 ng/mL

3.13-200 ng/mL

This kit recognizes 25-HVD3(25-Hydroxy Vitamin D3) in samples. No significant cross-reactivity or interference between 25-HVD3(25-Hydroxy Vitamin D3) and analogues was observed.

Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.

A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.

Store at 2‑8 °C.

This kit is only for scientific research, not for use