Human C5a ELISA Kit

ELISA

For the determination of Human C5a(Complement Component 5a) concentrations in serum, plasma and other related biological fluids.

This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human C5a(Complement Component 5a) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human C5a(Complement Component 5a) antibody. After incubation, add Biotin-conjugated anti-Human C5a(Complement Component 5a) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human C5a(Complement Component 5a) in the sample was proportional to the OD value. The concentration of Human C5a(Complement Component 5a) in the sample can be calculated by drawing a standard curve.

46.88 pg/mL

78.13-5000 pg/mL

This kit recognizes Human C5a(Complement Component 5a) in samples. No significant cross-reactivity or interference between Human C5a(Complement Component 5a) and analogues was observed.

Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.

A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.

Store at 2‑8 °C.

This kit is only for scientific research, not for use