



| Catalog No. | TD-VK794012 |
|---|---|
| Description | 3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage. |
| Expression system | E. coli |
| Protein length | 3C Protease is cloned from Human rhinovirus and expressed in E.coli. |
| Applications | Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP). |
| Species | HRV-14 |
| Predicted molecular weight | 47.46 kDa |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Form | Liquid |
| Storage buffer | 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Note | For research use only. |
|
SDS-PAGE for 3C Protease |
||||||||||||||||||
|
M: Marker
Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation,take 2–10 µg of the control and digested sample for analysis by SDS-PAGE. |
||||||||||||||||||
