TD-HF778023

Human

IF, IP, WB

Mouse

IgG2a, kappa

Y13-259

Monoclonal

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P01112

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

WB

1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).　　|　　Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog No.:TD-HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.　　|　　Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human KRAS/K-Ras 2 (Catalog No.:TD-HF904011), 50 ngLane 2:recombinant human KRAS/K-Ras 2 (Catalog No.:TD-HF904011), 30 ng

For research use only.