E. coli - derived recombinant Human SEMA3A (Lys26-His276).
Target
Semaphorin III, Sema III, SEMA3A, Semaphorin-3A, SEMAD
Purification
Purified by antigen affinity column.
Accession
Q14563
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | IHC | WB
Dilution
1:5000-1:20000 | 1:50-1:500 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caption
Western blot analysis was performed using anti-SEMA3A polyclonal antibody at 1ug/mL on various samples.Lane 1:C2C12 cell lysateLane 2:A549 cell lysateLane 3:HT-29 cell lysateLane 4:Raji cell lysateLane 5:Mouse spleen lysateLane 6:Mouse skeletal muscle lysateLane 7:Rat spleen lysate