E. coli - derived recombinant Human AXIN1 (Ser653-Asp862).
Target
AXIN, Axin-1, hAxin, Axis inhibition protein 1, AXIN1
Purification
Purified by antigen affinity column.
Accession
O15169
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | IHC | WB
Dilution
1:5000-1:20000 | 1:50-1:500 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caption
A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting AXIN1 (TD-HP232014) at a concentration of 13.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of AXIN1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
抗体
试剂盒
多肽
蛋白
组织芯片
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