TD-HV095014

Human, Mouse, Rat, Bos taurus, Macaca fascicularis, Pongo abelii

ELISA, IHC, WB

Rabbit

IgG

Polyclonal

E. coli - derived recombinant Human CACYBP (Ala2-Phe228).

S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein

Purified by antigen affinity column.

Q9HB71

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA　|　IHC　|　WB

1:5000-1:20000　|　1:50-1:500　|　1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (TD-HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).　|　Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples.Lane 1:K562 cell lysateLane 2:HCT116 cell lysateLane 3:MCF7 cell lysateLane 4:293T cell lysateLane 5:HepG2 cell lysateLane 6:HL60 cell lysateLane 7:Jurkat cell lysateLane 8:Rat brain lysateLane 9:Rat liver lysate

For research use only.