E. coli - derived recombinant Human U2AF1 (Met1-Arg196).
Target
U2AFBP, U2 auxiliary factor 35 kDa subunit, U2AF1, U2 snRNP auxiliary factor small subunit, U2 small nuclear RNA auxiliary factor 1, Splicing factor U2AF 35 kDa subunit, U2AF35
Purification
Purified by antigen affinity column.
Accession
Q01081
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | IHC | WB
Dilution
1:5000-1:20000 | 1:50-1:500 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caption
Western blot analysis was performed using anti-U2AF1 polyclonal antibody at 1μg/ml on various samples.Lane 1:Raji cell lysateLane 2:293T cell lysateLane 3:K562 cell lysateLane 4:Jurkat cell lysateLane 5:Mouse kidney lysateLane 6:Rat kidney lysate