Circular RNA CLASP1 modulates the GLI1/SNAIL axis and enhances macrophage polarization in breast cancer.[HY215014]
Catalog No.
TD-HY215014
Species reactivity
Human, Mouse, Rat, Bos taurus, Ovis aries, Pig
Applications
ELISA, IHC, WB
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CDK4 (Met1-Glu303).
Target
Cell division protein kinase 4, PSK-J3, Cyclin-dependent kinase 4, CDK4
Purification
Purified by antigen affinity column.
Accession
P11802
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | IHC | WB
Dilution
1:5000-1:20000 | 1:50-1:500 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caption
Western blot analysis of CCND1 and CDK4 protein levels in MDA-MB-231 and MCF-7 cells transfected with sh-circCLASP1 or sh-scramble.,Western blot analysis was performed using anti-CDK4 polyclonal antibody at 1ug/mL on recombinant CDK4 (Catalog No.:TD-HY215012).