TD-HY519014

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

IgG

Polyclonal

E. coli - derived recombinant Human ALDOC (Asp34-Ser281).

Brain-type aldolase, ALDOC, ALDC, Fructose-bisphosphate aldolase C

Purified by antigen affinity column.

P09972

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA　|　IHC　|　WB

1:5000-1:20000　|　1:50-1:500　|　1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALDOC (TD-HY519014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALDOC was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).　|　Western blot analysis was performed using anti-ALDOC polyclonal antibody at 1μg/ml on various samples.Lane 1:MCF7 cell lysateLane 2:LO2 cell lysateLane 3:k562 cell lysateLane 4:Jurkat cell lysateLane 5:Mouse brain lysateLane 6:Mouse kidney lysateLane 7:Mouse muscle lysateLane 8:Mouse heart lysate

For research use only.