E. coli - derived recombinant Human HSPA8 (Asp534-Gly615).
Target
Lipopolysaccharide-associated protein 1, HSPA10, Heat shock 70 kDa protein 8, LPS-associated protein 1, LAP-1, HSP73, HSC70, HSPA8, Heat shock cognate 71 kDa protein
Purification
Purified by antigen affinity column.
Accession
P11142
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | IHC | WB
Dilution
1:5000-1:20000 | 1:50-1:500 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caption
Western blot analysis was performed using anti-HSPA8 polyclonal antibody at 1ug/mL on various samples.Lane 1:4T1 cell lysateLane 2:MCF-7 cell lysateLane 3:PC-12 cell lysateLane 4:NIH3T3 cell lysateLane 5:Hela cell lysateLane 6:U87 cell lysateLane 7:Jurkat cell lysateLane 8:HepG2 cell lysateLane 9:A549 cell lysateLane 10:Mouse brain lysateLane11:Mouse heart lysateLane12:Rat brain lysateLane13:Rat heart lysate