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CD220
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ELISA
WB
IF
- Catalog： TD-32220
- Clonality： mAb
- Application： WB, FCM, IF
- Synonyms： INSR; HHF5
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规格：
100ul 50ul
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价格：
¥2180
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Mouse Monoclonal Antibody to CD220
| Description | |
| This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. |
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| Specification | |
| Aliases | INSR; HHF5 |
| Entrez GeneID | 3643 |
| Swissprot | P06213 |
| clone | 2A4H9 |
| WB Predicted band size | 156kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Monkey |
| Immunogen | Purified recombinant fragment of human CD220 (AA: extra 413-624) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
| WB | 1/500 - 1/2000 |
| IF | 1/50 - 1/200 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
| Product Image | |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using CD220 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), Hela (4), THP-1 (5), NIH/3T3 (6), COS7 (7), and PC-3 (8) cell lysate.
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Immunofluorescence analysis of Hela cells using CD220 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of THP-1 cells using CD220 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of HL-60 cells using CD220 mouse mAb (green) and negative control (red).
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