D12 is a fully human monoclonal antibody targeting PD-1, isolated from the synthetic AMG phage display library. It binds a novel epitope on PD-1 and induces an unusual conformational change at the receptor’s N-terminus. Despite limited overlap with the natural ligand-binding site, D12 effectively blocks PD-1 interaction with PD-L1 and PD-L2 through steric hindrance. The antibody shows high-affinity binding, recognizes PD-1 on activated human T cells, and cross-reacts with Cynomolgus monkey PD-1.
Species reactivity
Human
Applications
ELISA, Neutralization
Host species
Mouse
Isotype
IgG2a
Clone ID
D12
Clonality
Monoclonal
Target
Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q15116
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
SDS-PAGE for InVivoMAb Anti-Human CD279/PDCD1/PD1 Antibody (D12).
Note
For research use only. Not suitable for clinical or therapeutic use.
抗体
试剂盒
多肽
蛋白
组织芯片
定制服务咨询