TD-HV233013

Human

ELISA, FCM, IF, IP

Mouse

IgG2a

SAA0199

Monoclonal

Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q9Y6K1

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA

1:2000-1:20000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog No.:TD-HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog No.:TD-HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog No.:TD-HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog No.:TD-HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human DNMT3A IP Antibody (SAA0199).　　|　　Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human DNMT3A monoclonal antibody (Catalog No.:TD-HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human DNMT3A monoclonal antibody (Catalog No.:TD-HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

For research use only.