TD-HV675013

Human

Blocking, ELISA, FCM, SPR

Mouse

IgG2a, lambda

3H9

Monoclonal

A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, 3.4.24.87, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8, UNQ6102/PRO20085

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q76LX8

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA

1:2000-1:20000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SDS-PAGE for Anti-Human ADAMTS13 Antibody (3H9).　　|　　Flow-cytometry using anti-human ADAMTS13 antibody. ADAMTS13 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog No.:TD-HV675013, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human ADAMTS13 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog No.:TD-HV675013, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC- conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　Flow-cytometry using PE anti-human ADAMTS13 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAMTS13 monoclonal antibody (Catalog No.:TD-HV675013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

For research use only.