TD-HX855013

Human

ELISA, FCM, IHC, WB

Human

IgG1

005-C01

Monoclonal

CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P60033

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA　|　WB

1:2000-1:20000　|　1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SEC-HPLC detection for Anti-Human CD81 Antibody (005-C01).　　|　　Detects CD81 in indirect ELISAs.　　|　　Flow-cytometry using anti-human CD81 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog No.:TD-HX855013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human CD81 Antibody (005-C01).　　|　　Western blot analysis was performed using anti-CD81 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD81 (Catalog No.:TD-HX855011)Lane 2:negative control

For research use only.