TD-HY276013

Human

FCM, ICC, IF, WB

Mouse

IgG1, kappa

AF5

Monoclonal

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P02771

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

WB

1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SEC-HPLC detection for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).　　|　　Flow-cytometry using anti-human AFP antibody.AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog No.:TD-HY276013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).　　|　　Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog No.:TD-HY276013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.　　|　　Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human AFP/Alpha-fetoprotein (Catalog No.:TD-HY276011)Lane 2:negative control

For research use only.