Dermatophagoides farinae (American house dust mite), Dermatophagoides pteronyssinus (European house dust mite)
Applications
ELISA, WB
Host species
Human
Isotype
IgG1, kappa
Clone ID
4C1
Clonality
Monoclonal
Target
Peptidase 1, 3.4.22.65, Allergen Der f I, Major mite fecal allergen Der f 1, Der f 1, DERF1, Allergen Der p I, Major mite fecal allergen Der p 1, Der p 1, DERP1, Dermatophagoides farinae
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P16311 & P08176
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | WB
Dilution
1:2000-1:20000 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
Detects Dermatophagoides farinae (American house dust mite), Dermatophagoides pteronyssinus (European house dust mite) DERF1/DERP1 in indirect ELISAs. | SDS-PAGE for Anti-Dermatophagoides farinae DERF1/DERP1 Antibody (4C1). | Western blot analysis was performed using anti-American house dust mite DERF1 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant American house dust mite Der f 1/DERF1 (Catalog No.:TD-JN021011)Lane 2:negative control