Protein A/G purified from cell culture supernatant.
Accession
P32302
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Bioactivity
SEC-HPLC detection for Anti-Human CD185/CXCR5 Antibody (16D7).
Flow Cytometry
Flow-cytometry using anti-Human CD185/CXCR5 antibody. 293T cells transfected with CD185/CXCR5 (Red Histogram) or Empty vector (Yellow Histogram) with an anti-human CD185/CXCR5 antibody monoclonal antibody (Catalog #TD-HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF647 conjugated Goat anti-Mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human CD185 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD185 monoclonal antibody (Catalog # TD-HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog #TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.