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Anti-Human CD33 Antibody (SAA1404)
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Anti-Human CD33 Antibody (SAA1404)
| Catalog No. | TD-HB185307 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG2a, kappa |
| Clone ID | SAA1404 |
| Clonality | Monoclonal |
| Target | Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P20138 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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SEC-HPLC detection for Anti-Human CD33 Antibody . |
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Flow-cytometry using anti-human CD33 antibody.CD33 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # TD-HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD33 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # TD-HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD33 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD33 monoclonal antibody (Catalog # TD-HB185307) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD33 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 monoclonal antibody (Catalog # TD-HB185307, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |