TD-HV985207

Anti-Human NECTIN4/PVRL4 Antibody (SAA0403)(TD-HV985207) is amouse monoclonalantibody detectingLNIRinELISA, FCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

ELISA, FCM

Mouse

IgG2a, kappa

SAA0403

Monoclonal

LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q96NY8

Liquid

0.01M PBS, pH 7.4, 0.09% Sodium azide.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Detects NECTIN4/PVRL4 in indirect ELISAs.　　|　Flow-cytometry using anti-human NECTIN4 antibody.NECTIN4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NECTIN4 antibody monoclonal antibody (Catalog No.:TD-HV985207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human NECTIN4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human NECTIN4 monoclonal antibody (Catalog No.:TD-HV985207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human NECTIN4 (Catalog No.:TD-HV985011)Lane 2:negative control

For research use only.