TD-VK425011

Recombinant CHIKV Spike glycoprotein E1 Protein, C-Fc(TD-VK425011) expressed inMammalian Cells, spanningTyr810-Lys1221. Purity:>90%by SDS-PAGE.HighlightsFc Fusion— Enhanced stability with Protein A/G purification compatibility.Mammalian Expression— Proper folding and native glycosylation.High Purity— >90% purity verified by SDS-PAGE.

Mammalian Cells

Q8JUX5

Tyr810-Lys1221

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Chikungunya virus (strain S27-African prototype) (CHIKV)

Recombinant

Please contact with the lab for this information.

>90% as determined by SDS-PAGE.

73.11 kDa

Lyophilized

Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.　　RECONSTITUTION PROTOCOL　　1.　Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. ２.　Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. ３.　Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. ４.　After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial.Note: Do not mix by vortexing or by pipetting the material up and down. ５.　Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. ６.　Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for Chikungunya fever, a disease marked by fever, rash, and joint pain. The E1 envelope glycoprotein, a 47 kDa class II fusion protein, plays a critical role in viral entry by mediating low pH-triggered membrane fusion within host endosomes. It forms heterodimers with E2 glycoproteins, assembling into spike trimers on the viral surface that facilitate host cell attachment and infection. Due to its essential function in viral infectivity, the E1 protein is widely used in studies of viral entry, antibody development, vaccine design, and diagnostic assay optimization.

SDS-PAGE for Recombinant CHIKV Spike glycoprotein E1 Protein, C-Fc

For research use only.