TD-HB167023

Human

ELISA, FCM

Mouse

IgG2a, kappa

M3/38

Monoclonal

Mac-2 antigen, Lectin L-29, LGALS3, Galactose-specific lectin 3, 35 kDa lectin, Galactoside-binding protein, CBP 35, L-31, Gal-3, Laminin-binding protein, Galectin-3, GALBP, IgE-binding protein, Carbohydrate-binding protein 35, MAC2

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P17931

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA

1:2000-1:20000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Detects Human LGALS3 in indirect ELISAs.　　|　　Flow-cytometry using anti-human Galectin-3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog No.:TD-HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human Galectin-3 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog No.:TD-HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human Galectin-3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog No.:TD-HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human Galectin-3 antibody.SiHa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog No.:TD-HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human Gal3/Galectin-3 Antibody (M3/38).　　|　　Flow-cytometry using anti-human Galectin-3 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 monoclonal antibody (Catalog No.:TD-HB167023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.