Caption
Western blot analysis was performed using anti-TSG101 monoclonal antibody at 1ug/mL on various samples.Lane 1:SH-SY5Y cell lysateLane 2:K562 cell lysateLane 3:NIH3T3 cell lysateLane 4:PC-12 cell lysateLane 5:Lncap cell lysateLane 6:293T cell lysateLane 7:Mouse brain lysateLane 8:Rat brain lysateLane 9:Rat heart lysate | Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog No.:TD-HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog No.:TD-HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.