TD-HT173013

Human

Blocking, ELISA, FCM

Mouse

IgG2a, kappa

G9.2-17

Monoclonal

Ecalectin, LGALS9, Galectin-9, Gal-9, Tumor antigen HOM-HD-21

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

O00182

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA

1:2000-1:20000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human Galectin-9 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog No.:TD-HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human Galectin-9 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog No.:TD-HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human Galectin-9 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog No.:TD-HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human Gal-9/LGALS9 Antibody (G9.2-17).　　|　　Flow-cytometry using anti-human Galectin-9 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 monoclonal antibody (Catalog No.:TD-HT173013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.