TD-HV388025

Human

ELISA, FCM, WB

Mouse

IgG1

1A209

Monoclonal

Recombinant Human FOXP3 protein.

Forkhead box protein P3, Scurfin, Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, FOXP3, IPEX, JM2

Protein A/G purified from cell culture supernatant.

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog No.:TD-HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog No.:TD-HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog No.:TD-HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog No.:TD-HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.