TD-HY436013

Human

WB

Mouse

IgG2a, kappa

2A2

Monoclonal

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P07858

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

WB

1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SDS-PAGE for Anti-Human CTSB Antibody (2A2).　　|　　Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog No.:TD-HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.　　|　　Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human CTSB (Catalog No.:TD-HY436012), 50ngLane 2:recombinant human CTSB (Catalog No.:TD-HY436012), 30ngLane 3:recombinant human CTSB (Catalog No.:TD-HY436012), 20ng

For research use only.