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Anti-Human EGFR/ERBB1/HER1 Antibody (11F8)
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| Catalog No. | TD-HF004107 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM, WB |
| Host species | Human |
| Isotype | IgG1, kappa |
| Clone ID | 11F8 |
| Clonality | Monoclonal |
| Target | HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P00533 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Flow-cytometry using anti-human EGFR antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # TD-HF004107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human EGFR antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # TD-HF004107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human EGFR antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # TD-HF004107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer. |