TD-HV456107

Anti-Human CD112/Nectin-2/PVRL2 Antibody (SAA0405)(TD-HV456107) is amouse monoclonalantibody detectingHerpesvirus entry mediator BinELISA, FCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

ELISA, FCM

Mouse

IgG2a, kappa

SAA0405

Monoclonal

Herpesvirus entry mediator B, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, HVEB, PVRL2 receptor-related protein 2, CD112, PRR2, Nectin-2, HveB, NECTIN2

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q92692

Liquid

0.01M PBS, pH 7.4, 0.09% Sodium azide.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Detects CD112/Nectin-2/PVRL2 in indirect ELISAs.　　|　Flow-cytometry using anti-human Nectin2 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog No.:TD-HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human Nectin2 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog No.:TD-HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD112 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD112 monoclonal antibody (Catalog No.:TD-HV456107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Western blot analysis was performed using anti-CD112/Nectin-2/PVRL2 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD112/Nectin-2/PVRL2 (Catalog No.:TD-HV456011)Lane 2:negative control

For research use only.