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Anti-Human CD126/IL6R/IL-6RA Antibody (SAA0048)
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| Catalog No. | TD-HY583107 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Clone ID | SAA0048 |
| Clonality | Monoclonal |
| Target | sIL6R, Interleukin-6 receptor subunit alpha, CD126, IL-6R 1, IL-6R subunit alpha, Membrane glycoprotein 80, gp80, IL6R, IL-6RA, IL-6 receptor subunit alpha, IL-6R-alpha |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P08887 |
| Form | Liquid |
| Storage buffer |
0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Flow-cytometry using anti-human CD126 antibody.CD126 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # TD-HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD126 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # TD-HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD126 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 monoclonal antibody (Catalog: TD-HY583107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Western blot analysis was performed using anti-CD126/IL6RA monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD126/IL6RA (Catalog No: TD-HY583011) Lane 2: negative control |